alexa fluor 647 goat anti-mouse igg2a a-21241  (Thermo Fisher)

Journal: Frontiers in chemical biology

Article Title: An in vivo BSL-2 model for henipavirus infection based on bioluminescence imaging of recombinant Cedar virus replication in mice

doi: 10.3389/fchbi.2024.1363498

Figure Lengend Snippet: Mice were mock-infected or infected with 107 PFU of rCedV-luc via the I.P. route. (n = 5 mock-infected IFNAR-KO females; 6 infected albino B6 females; 6 infected albino IFNAR-KO (2 females, 4 males)). Blood was collected via terminal bleed at day 21 post-infection, and analyzed for CedV G reactivity using a Luminex bead-based assay. Isotype/subtype-specific antibodies were used to semi-quantitatively assess amounts of total IgG, IgG1, IgG2b, IgG3 and IgM. See Table 3 for per animal numerical values. The Kruskal-Wallis test with Dunn’s correction for multiple comparisons was used to determine significance in comparisons between albino B6 vs. mock and albino IFNAR-KO vs. mock. For mock-infected vs. albino B6, p values were: Total IgG, 0.069; IgG1, 0.436; IgG2b, 0.111; IgG3, >0.999; IgM, 0.265. For mock-infected vs. albino IFNAR-KO, p values were: Total IgG, 0.002; IgG1, 0.005; IgG2b, 0.0098; IgG3, 0.006; IgM, 0.731. Error bars are SD.

Article Snippet: To detect CedV, sections were stained with mouse anti-CedV N (clone 1D5, 1:10, generated by GenScript Biotech (Piscataway, NJ) via contracted production) overnight at 4°C, followed by washing and secondary staining with Alexa Fluor 647 goat anti-mouse IgG2a (Thermo Fisher cat #A-21241, 1:1000) for 1 h at ambient temperature.

Techniques: Infection, Luminex, Bead-based Assay